Expression of Myc-tagged PfCLKs in blood stages. Indirect immunofluorescence assay using anti-Myc antibody showed a distinct punctate protein expression of PfCLK-1-Myc in association with the nucleus of trophozoites (TZ) and schizonts (SZ). PfCLK-2-Myc localizes in the nucleus and cytoplasm of blood stage parasites. Kinase labelling was visualized with AlexaFluor488 secondary antibody (green), nuclei were highlighted by Hoechst staining (blue), red blood cells (RBC) were counterstained with Evans Blue (red). Bar, 5 μm. Immunofluorescence assay on methanol-fixed mutants, using anti-Myc antibody, showed punctate expression of PfCLK-1-Myc in the nuclei of trophozoites and schizonts, while PfCLK-2-Myc was present in the nuclei and the cytoplasm of blood stages

Agarwal S, Kern S, Halbert J, Przyborski JM, Baumeister S, Dandekar T, Doerig C, Pradel G. Two nucleus-localized CDK-like kinases with crucial roles for malaria parasite erythrocytic replication are involved in phosphorylation of splicing factor. J Cell Biochem. 2011 112:1295-310.