Left: PfRON2 is expressed at the apical end of Plasmodium merozoites. Schizont infected erythrocytes and merozoites were dual-labeled with antisera against PfRON2 and PfAMA1 PF11_0344 (A), PfClag3.1 PFC0110w (B), or PfRON4 PF11_0168 (C). Merged images are shown in the right panels. All segmented schizonts and merozoites are positive for PfRON2. Nuclei are counterstained with DAPI. Colocalization of PfRON2 with PfRON4 (rhoptry neck marker) was observed but neither colocalized with PfClag3.1 (rhoptry body marker) nor PfAMA1 (microneme marker). Right: Longitudinally sectioned merozoites in schizont-infected erythrocytes were labeled with anti-PfRON2 serum followed by secondary Ab conjugated with gold particles. Gold particles were restricted to the narrow neck portion of the rhoptries (R). Two different images are shown (A and B). N indicates nucleus. Bars=200 nm.
Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Gao Q, Tsuboi T, Torii M. Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites. Parasitol Int. 2009 58:29-35.
Other associated proteins
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1452000||rhoptry neck protein 2|