PF3D7_1452300 DER1-like protein

In vivo localization of the Plasmodium falciparum Localization of the symbiont-specific Der1-1 BTS–GFP fusion protein in P. falciparum 3D7 blood stage parasites (3D7sDer1-1 BTSGFP ; in green) additionally stained with MitoTracker (in red) and Hoechst (in blue). The typical ‘‘dot’’ pattern of apicoplast targeted GFP is clearly evident. Scale bar represents 4 mm. The BTS of the P. falciparum symbiont Der1-1 (3D7sDer1-1 BTSGFP) is able to direct GFP to the apicoplast BTS - bipartite topogenic signal, comprised of an N-terminal signal peptide and a plastid transit peptide, the sequence of which determines whether 2 membranes (into the PPC) or 4 membranes (into the plastid stroma) are crossed. Staining with Hoechst and MitoTracker proves that the GFP expression does not display a nuclear or mitochondrial localization.

Sommer MS, Gould SB, Lehmann P, Gruber A, Przyborski JM, Maier UG. Der1-mediated preprotein import into the periplastid compartment of chromalveolates? Mol Biol Evol. 2007 24(4):918-28

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