Localization of PfAPP by cryoimmunoelectron microscopy. Parasitized erythrocytes were fixed with 4% paraformaldehyde and 0.1% glutaraldehyde and were labeled with anti-PfAPP serum (1:2,500 dilution). rbc, red blood cell; fv, food vacuole; pvm/ppm, parasitophorous vacuole membrane/parasite plasma membrane. Bar, 200 nm. The distribution of gold particles was 79 % in the food vacuole and 21 % outside of the food vacuole but within the parasite.
Ragheb D, Bompiani K, Dalal S, Klemba M. Evidence for catalytic roles for Plasmodium falciparum aminopeptidase P in the food vacuole and cytosol. J Biol Chem. 2009 284(37):24806-15 PubMed