Indirect immunofluorescence analysis of V-H+-PPase in trophozoites of P. falciparum. Fluorescence (A, B, C) or bright field (D, E, F) images of infected erythrocytes: A,D, with preimmune serum; B,E, with antibody 324; C,F, with antibody 326. The images show intense labeling over the whole parasites and bright, intracellular spots. Arrows show trophozoite stages of the parasite. Bars=10 mm. Immunofluorescence showed a general fluorescence over the whole parasites and intracellular bright spots suggesting a vesicular and plasma membrane localization.
Luo S, Marchesini N, Moreno SN, Docampo R. A plant-like vacuolar H+-pyrophosphatase in Plasmodium falciparum. FEBS Lett. 1999 460:217-20.