FCP localizes to the lumen of the FV. A, cryo-immunoelectron micrograph showing a close-up of a folded FV with immunogold-stained FCP in the lumen of the parasite FV in a late trophozoite. Various structures are indicated as host red blood cell cytosol (RBC Cytosol), parasite body (parasite), parasite nucleus (Nu), FV, and hemozoin (Hz). B shows a more complete parasite with a cross-section through the FV indicating a lack of immunogold staining outside the FV. C, three parasites from a different region of the same ultrathin section that was immunogold-stained for anti-FCP in A and B. Note the lack of concentrated gold particles on all visible parasite structures including ER, parasite plasma membrane (PPM), parasitophorous vacuolar membrane (PVM), and hemoglobin containing vesicles (HV).

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