Immunolocalisation of (A) glycophorin A and B by monoclonal antibodies (purchased from Sigma, St. Louis, USA) and (B) EXP-2 using a specific antiserum was performed with mature iRBCs incubated with 10 mM bADA for 10 h prior to fixation and electronmicroscopy. (C,D) Enlarged regions of (A) and (B), respectively, as indicated by frames. Immunogold labeling is indicated by arrowheads. EXP-2 is a parasite protein of the PVM. During merozoite release, it became apparent that the merozoites were enveloped by the red blood cell plasma membrane. However, the PVM was found to be lysed; fragments of the PVM were found inside the clusters between the merozoites as membranous whorls.
Gelhaus C, Vicik R, Schirmeister T, Leippe M. Blocking effect of a biotinylated protease inhibitor on the egress of Plasmodium falciparum merozoites from infected red blood cells. Biol Chem. 2005 386:499-502. Copyright Walter de Gruyter GmbH 2009.