Indirect immunofluorescence using P. falciparum-infected erythrocytes glutaraldehyde fixed in suspension only (a) or subsequently treated with 0.1% saponin (b) or 10% Triton X-100 (c, d). It was previously shown that Pfl55/RESA is not accessible for reaction with antibodies on the surface of intact P. falciparum-infected erythrocytes unless the membrane is modified, e.g., by glutaraldehyde fixation and air dryingProbing was done with the human MAb 3362 (a-c) or the mouse Mab 4C8F6 directed against a cytoplasmic epitope of band 3 (d). The samples were counterstained with ethidium bromide to visualize the intracellular parasites.

Ruangjirachuporn W, Udomsangpetch R, Carlsson J, Drenckhahn D, Perlmann P, Berzins K. Plasmodium falciparum: analysis of the interaction of antigen Pf155/RESA with the erythrocyte membrane. Exp Parasitol. 1991 73:62-72. Copyright Elsevier 2010.