Parasites were tagged by single cross-over homologous recombination, the endogenous gene locus to include the GFP coding sequence. PFA660INT. Co-immunofluorescence analysis on erythrocytes infected with PFA660INT, using anti-sera directed against STEVOR (A), MAHRP1 MAL13P1.413 (B), KAHRP PFB0100c (C). Fluorescence channels are shown individually in black/white for highest contrast. All images are maximal projections of Z-stack serial sections. In merge image green, GFP; red, STEVOR (A), MAHRP (B), KAHRP (C) or ATS domain of PfEMP1 (D); blue, Hoechst. Inset shows enlargement of merge (white box). The fusion protein labelled punctate structures within the infected erythrocytebut clearly not in Maurer’s clefts.
Külzer S, Rug M, Brinkmann K, Cannon P, Cowman A, Lingelbach K, Blatch GL, Maier AG, Przyborski JM. Parasite encoded Hsp40 proteins define novel mobile structures in the cytosol of the P. falciparum infected erythrocyte. Cell Microbiol. 2010 12(10):1398-420. Copyright John Wiley & Sons Ltd. 2010.
Other associated proteins
|PF3D7_0113700||heat shock protein 40, type II|
|PF3D7_1370300||membrane associated histidine-rich protein|