(A) live cell fluorescence microscopy of 3D7-KAHRP-GFP. Panels are GFP fluorescence, bright field (BF) and a merge of the two. Scale bar: 5 μm. KAHRP-GFP was trafficked to the RBC membrane and gave a rim fluorescence pattern (B) Immunofluorescence microscopy of acetone-fixed RBCs infected with 3D7-KAHRP-GFP, SBP1 is a marker for the Maurer’s clefts.
Lower row: Immunofluorescence microscopy of acetone fixed RBCs infected with wild type. Smears were probed with mouse anti-PfEMP3 (green) and rabbit anti-SBP1 (red). A characteristic PfEMP3 profile at the RBC membrane is seen.
Dixon MW, Kenny S, McMillan PJ, Hanssen E, Trenholme KR, Gardiner DL, Tilley L. Genetic ablation of a Maurer's cleft protein prevents assembly of the Plasmodium falciparum virulence complex. Mol Microbiol. 2011 81(4):982-93.
Other associated proteins
|PF3D7_0201900||erythrocyte membrane protein 3|
|PF3D7_0202000||knob-associated histidine-rich protein|