Stage analysis of HRPl (KAHRP) and PfEMP3 synthesis in PE by IFA and confocal microscopy. Both the MC K+ and the FCR-3 K+ parasites were analyzed, each producing the same fluorescent patterns. HRPl was detected with mAb89 and PfEMP3 was detected using chicken anti-PfEMP3 polyclonal antibody. The images in the first (fluorescent) and second (transmitted light) columns are using anti-HRPl antibody. The images in the third (fluorescent) and fourth (transmitted light) columns are using anti-PfEMP3 antibody. Panels A and B, ring stage (12-15 h); panels C and D, early- to mid-trophozoite stage (24-30 h); panels E and F, schizont stage (40-48 h). The arrowheads designate the erythrocyte membrane of the parasitized eryhtrocyte.
Pasloske BL, Baruch DI, Ma C, Taraschi TF, Gormley JA, Howard RJ. PfEMP3 and HRP1: co-expressed genes localized to chromosome 2 of Plasmodium falciparum. Gene. 1994 144:131-6. Copyright Elsevier 2010.
Other associated proteins
|PF3D7_0201900||erythrocyte membrane protein 3|