KAHRP protein trafficking in PEs. Bright field (BF) and GFP fluorescence images of PEs containing Pf 3D7 parasites transformed to express KAHRP(+His)-GFP protein. Scale bar represents 5 nm. Fluorescence from the tagged KAHRP first appeared within ring stage parasites and was associated with the PVM. Progression to trophozoites was accompanied by the appearance of fluorescent spots in the host erythrocyte cytoplasm often near the host membrane. These fluorescent concentrates of protein have been shown to be associated with Maurer’s clefts. In later trophozoite stages, concentrates of protein and an increased component of fluorescence were associated with the erythrocyte membrane .
Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems, TE. Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes. PLoS ONE 2011, 6:e22975