PfHRP-1 is now named KAHRP (Knob associated histidine-rich protein). Immunoelectron microscopy on cryo-thin section of K ÷ P. falciparum-infected erythrocytes cultured in vitro 32 h and then treated with mAb 89 and protein A-gold. (a) PfHRP-1 is not seen within the parasite (P) but is found external to the parasite membrane (M) and in association with the parasitophorous vacuolar membrane (V). (b) PfI-IRP-1 is seen in membranous vesicles and (c, d) in electron dense granules in the erythrocyte cytosol. (d, e) PfHRP-1 can be seen approaching the erythrocyte membrane. Arrow identifies the erythrocyte surface (S). Bars represent 0.1 mm.
Taylor DW, Parra M, Chapman GB, Stearns ME, Rener J, Aikawa M, Uni S, Aley SB, Panton LJ, Howard RJ. Localization of Plasmodium falciparum histidine-rich protein 1 in the erythrocyte skeleton under knobs. Mol Biochem Parasitol. 1987 25:165-74. Copyright Elsevier 2009.