Colocalization of malarial and host proteins in the erythrocyte membrane. A blood smear of erythrocytes infected with the trophozoite stage parasites was fixed and then reacted with two different antibodies (from rabbit and from mouse) against the proteins under study. Secondary antibodies labeled with FITC (anti-rabbit) and TR (anti-mouse) were used, respectively. The left column shows the two simultaneously acquired fluorescence channels by NSOM (near-field scanning optical microscope) dualcolor imaging. The center column presents the corresponding overlay of the fluorescence images (a) Control experiment: primary antibodies against KAHRP. (b) Colocalization of MESA and protein 4.1 (a host cell membrane protein). (c) Colocalization of KAHRP and protein 4.1. Scale bars 5 2 mm.
Enderle T, Ha T, Ogletree DF, Chemla DS, Magowan C, Weiss S. Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy. Proc Natl Acad Sci U S A. 1997 94:520-5.
Other associated proteins
|PF3D7_0202000||knob-associated histidine-rich protein|