PF3D7_0207600 serine repeat antigen 5

SBP1 (SERA5 targeting peptide) colocalizes with SERA5 within the parasitophorous vacuole of mature blood-stage parasites. Late trophozoites (A) or schizonts (B) were probed with biotinylated SBP1 (column 1; green) and MAb 3E8 (column 2; red). Parasite nuclei (column 3) were stained (blue) using the DNA stain Hoechst 33258. Areas of colocalization for SBP1 and SERA5 are observed as yellow areas (column 4) in the superimposed fluorescence patterns for biotinylated SBP1 and MAb 3E8. The transmission images of parasites viewed using light microscopy are shown in column 5. Bar, 2 mm. SBP1 sequence: LVCHPAVPALLCAR.

Lower panel: Immuno-EM images of synchronized parasites cultured in the presence of biotinylated SBP1 (710 M). Sections were probed with MAb 2F3 (to detect SERA5) and avidin (to detect biotinylated SBP1) and visualized using 18-nm and 10-nm gold beads, respectively. SERA5 was generally restricted to the parasitophorous vacuole.

Fairlie WD, Spurck TP, McCoubrie JE, Gilson PR, Miller SK, McFadden GI, Malby R, Crabb BS, Hodder AN. Inhibition of malaria parasite development by a cyclic peptide that targets the vital parasite protein SERA5. Infect Immun. 2008 76:4332-4344.

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