Expression of rhoptry protein CLAG3.1 on surface of P. falciparum merozoites isolated in intracellular buffer (5 mM NaCl, 140 mM KCl, 1 mM EGTA), detected by IFA using anti-CLAG3.1 rabbit sera followed by FITC-conjugated (green) anti-rabbit IgG goat sera. Nuclear DNA was counterstained with DAPI (blue). Bright field, bright field merged with DAPI staining and merged fluorescence images (DAPI and FITC) are shown. Black bar indicates 2 mm.
Singh S, Alam MM, Pal-Bhowmick I, Brzostowski JA, Chitnis CE. Distinct external signals trigger sequential release of apical organelles during erythrocyte invasion by malaria parasites. PLoS Pathog. 2010 Feb 5;6(2):e1000746.