The anti-phospho-CLAG 3.1 antibody was used to determine the localization of phospho-CLAG 3.1 by immunocytochemistry of paraformaldehyde-fixed schizont stage parasites. Shown are two examples of schizont (segmenter) parasites stained with the phospho-CLAG 3.1 antibody. The samples were also stained with 4,6-diamidino-2-phenylindole (blue) to label the nuclei. The scale bar indicates 2 μM. The experiments shown are representative of at least three independent experiments. This antibody identified a punctuate localization of phospho-CLAG 3.1 consistent with rhoptry localization within merozoites of a mature schizont.
Solyakov L, Halbert J, Alam MM, Semblat JP, Dorin-Semblat D, Reininger L, Bottrill AR, Mistry S, Abdi A, Fennell C, Holland Z, Demarta C, Bouza Y, Sicard A, Nivez MP, Eschenlauer S, Lama T, Thomas DC, Sharma P, Agarwal S, Kern S, Pradel G, Graciotti M, Tobin AB, Doerig C. Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum. Nat Commun. 2011 2:565.