PFC0435w is a junctional protein of the TVN. A) Relative distribution of PFC0435w-GFP (i) or HTTM-GFP (ii) and Maurer’s clefts marked by PfSBP1 (ii, iv) in P. falciparum infected erythrocytes.The cells were also stained with Hoechst 33342 (blue) to visualize the DNA of the parasite. Panels i, ii and iv, v are single optical sections, whose merge is shown in iii and vi respectively. Scale bar, 2 mm. B–D) Association of PFC0435w-GFP with membrane buds and TVN structures B. Zero degree projection of an erythrocyte infected with a PFC0435w- GFP-expressing parasite stained with Rhodamine B. The numbers indicate the large circular TVN structure (1), and PFC0435w-GFP associated with other parts of the TVN (2 and 3). C) Cartoon representation of the infected cell in panel B. D) Optical sectioning of the infected erythrocyte shown in panel B. Distance between sections is 0.2 mm. Note the progression of PFC0435w-GFP staining from the PVM (sections 1–4) to the circular Rhodamine B-stained structure in the erythrocyte (sections 4–8). Arrows indicate PVM buds colocalizing with PFC0435w-GFP. Arrowhead indicates TVN connections between two large loops.
van Ooij C, Tamez P, Bhattacharjee S, Hiller NL, Harrison T, Liolios K, Kooij T, Ramesar J, Balu B, Adams J, Waters AP, Janse CJ, Haldar K. The malaria secretome: from algorithms to essential function in blood stage infection. PLoS Pathog. 2008 Jun 13;4(6):e1000084,
Other associated proteins
|PF3D7_0310400||TVN-junction protein 1 parasite-infected erythrocyte surface protein|