Localization of Pfstomatin to the apical organelles of merozoites and association of parasite rhoptry protein RhopH2 . Indirect immunofluorescence assays of: (i–iii) free merozoites probed with antibodies to Pfstomatin (i; green), and Mito Tracker (ii; red); the relative lack of co-localization of these markers is shown in the merged panel iii. iv–vi intracellular schizont-stage parasites expressing GFP in the apicoplast were probed with antibodies to Pfstomatin (iv; red), GFP (v; green); the relative lack of co-localization of these markers is shown in the merged panel vi. vii–ix free merozoites probed with antibodies to: Pfstomatin (vii; green), RhopH2 (viii; red); the extent of co-localization is shown in the merged panel ix. All images shown are single optical sections. The nucleus (blue) was stained with Hoechst 33342. Scale bars correspond to 2 mm.

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