Immunoelectron micrographs depicting the association of GFP-PfSec22 with transition vesicles and tubovesicular elements. Ultrathin cryosections of the GFP-PfSec22 expressing cells were probed with monoclonal anti-GFP antibodies followed by immunogold detection using gold (12nm)-labeled anti-mouse secondary antibodies. (A) Association of GFP-PfSec22 with ER-derived transition vesicles (black arrowhead). (B) Association of GFP-PfSec22 with membrane-limited vesicles (white arrowheads) in the host cell compartment. (C) Association of GFP-PfSec22 with the TVN-like extension (dotted arrow) and membrane-bound vesicles (white arrowhead) in the infected 7 erythrocyte cytosol. N: nucleus, MC: Maurer’s cleft, RBC: red blood cell cytosol. Scale bars are 8 500 nm in (A), and 100 nm in (B) and (C).

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