Confocal immunofluorescence of PfSar1p. Panels A-C: Parasitized erythrocyes (D10 strain) were labeled with affinity purified anti=PfSar1p antiserum followed by fluorescein-conjugated anti-rabbit antiserum (green). Panels D-F: Transmission images of panels A-C. Panels G-L: Dual labeling of affinity-purified rabbit anti-PfSar1p antiserum followed by fluorescein-conjugated anti-rabbit antiserum (green) and a murine monoclonal antibody recognizing the parasitophorous vacuole-located protein, Exp-1, followed by rhodamine-conjugated anti-mouse antiserum (red). Sar1p in the trophozoite is clearly located in the erythrocyte cytosol (G-K) and in the schizont it occupies most of it (L). Panels M-O: Dual labeling of affinity-purified rabbit anti-PfSar1p antiserum followed by rhodamine-conjugated anti-mouse antiserum (red) and a biotin-conjugated anti-PfERC antiserum followed by fluorescein-conjugated anti-rabbit antiserum (green). PfERC and PfSar1p labeling patterns do not completely coincide, indicating that PfSar1p is not confined to the ER. An optical slice was collected through the center of the parasite by confocal microscopy. Some PfSar1p-containing structures in the erythrocyte cytosol are marked with white arrowheads. Some PfSar1p-containing structures in the parasite cytosol are marked with blue arrowheads. The bar in panel C corresponds to 5 mm.

Albano FR, Berman A, La Greca N, Hibbs AR, Wickham M, Foley M, Tilley L. A homologue of Sar1p localises to a novel trafficking pathway in malaria-infected erythrocytes. Eur J Cell Biol. 1999 78:453-462. Copyright Elsevier 2010.