Upper 2 rows: Localization of Pf34 to the rhoptries was demonstrated in late stage parasites by immunostaining infected blood smears with anti-Pf34 and co-localising with either anti-RAMA or anti-RhopH3 (both rhoptry markers) or anti-AMA-1 (microneme marker) antibodies.
Lower row: A mature schizont (i) and two extracellular merozoites (ii) showing the close apposition of Pf34-positive (green) and RAMA-positive (red) structures in the apex of the merozoite. Panels (iii) and (iv) show the apex of merozoites and the two rhoptries connected to the Pf34-positive structure at the anterior when visualised by confocal microscopy. N, nucleus; R, rhoptry. Bars represent 1 mm in (i) and (ii) and 500 nm in (iii) and (iv).
Proellocks NI, Kovacevic S, Ferguson DJ, Kats LM, Morahan BJ, Black CG, Waller KL, Coppel RL. Plasmodium falciparum Pf34, a novel GPI-anchored rhoptry protein found in detergent-resistant microdomains. Int J Parasitol. 2007 37:1233-41. Copyright Elsevier
Other associated proteins
|PF3D7_0419700||apical merozoite protein|
|PF3D7_0707300||rhoptry-associated membrane antigen|