Immunofluorescence detection of the RH5 protein in the P. falciparum FCB-2 strain using rabbit sera as primary antibody and fluorescein (FITC)-labelled anti-rabbit IgG as secondary antibody. (1) Negative control and (2) and (3) apical fluorescence. The antibodies recognized a protein having a point pattern on late-stage schizonts which is typical of apical organelles.
Arévalo-Pinzón G, Curtidor H, Muñoz M, Patarroyo MA, Bermudez A, Patarroyo ME. A single amino acid change in the Plasmodium falciparum RH5 (PfRH5) human RBC binding sequence modifies its structure and determines species-specific binding activity. Vaccine. 2011 30(3):637-46