Upper: Localization of MESA. Thin blood smears of mature trophozoite-infected erythrocytes were made from in vitro cultures, air-dried, and fixed with acetonelrnethanol. They were reacted at room temperature for 45 minutes with anti-MESA diluted 1:lOO in PES, washed 3 times in PES, and reacted for 45 minutes with fluorescein isothiocyanate-conjugated goat-antirabbit IgG diluted 1:lOO in PBS. Slides were again washed 3 times in PBS and mounted with SlowFade and a coverslip for examination with a confocal laser scanning microscope equipped with Nikon epifluorescence.

Lower: Localization of KAHRP. Cells were prepared and examined as above except that in this case they were reacted with MoAb 89 (antiknob-associated histidine-rich protein) diluted 120 in PBS, followed by fluorescein isothiocyanate-conjugated goat-antimouse IgG diluted 1:lOO in PBS.

Magowan C, Coppel RL, Lau AO, Moronne MM, Tchernia G, Mohandas N.

Role of the Plasmodium falciparum mature-parasite-infected erythrocyte surface antigen (MESA/PfEMP-2) in malarial infection of erythrocytes.

Blood. 1995 86:3196-204.