Confocal laser scanning IFA colocalization analyses of hPrx-2 in infected RBCs. (A) IFA colocalization of hPrx-2 (human peroxiredoxin 2) with cytosolic GFP in P. falciparum late schizont/ merozoites. The surrounding RBC is lysed and merozoites are about to be released. Phase contrast (PC) shows the residual hemozoin crystal and merozoites. DNA staining (blue) visualizes 28 merozoites. Cytosolic GFP (red) stains the merozoites and anti-hPrx-2-staining shows presence of hPrx-2 in merozoites (hPrx-2, green). ‘‘Overlay’’ shows a weak colocalization of GFP and hPrx-2. (B) IFA colocalization analysis of hPrx-2 in a trophozoite. PC shows the parasite and the FV containing hemozoin. Anti-hPrx-2-staining (green) reveals the presence of the protein in the trophozoite cytosol as it colocalizes with cytosolic GFP (red). (C and D) IFA colocalization of hPrx-2 with PfSBP1 in P. falciparum trophozoites. PfSBP1 (red) stains the MCs of the trophozoite. Anti-hPrx-2-staining shows presence of hPrx-2 in parasite cytosol and focal staining in the RBC (hPrx-2, green). ‘‘Overlay’’ shows colocalization of hPrx-2 and PfSBP1 in the Maurer’s Clefts.
Koncarevic S, Rohrbach P, Deponte M, Krohne G, Prieto JH, Yates J 3rd, Rahlfs S, Becker K. The malarial parasite Plasmodium falciparum imports the human protein peroxiredoxin 2 for peroxide detoxification. Proc Natl Acad Sci U S A.2009 106:13323-8. PMID: