A. Labeling of CLAG9 in 3D7 and E5KO. Note there is labeling of E5KO (clag9 knockout) rhoptries with mAb7H8/50 that reveals RAP3, but not with anti-CLAG9 specific sera due to gene disruption. Scale bars = 5 mm. B. Trafficking of PfEMP1 and KAHRP by E5KO; labeling was identical to 3D7. Scale bars = 5 mm. CLAG9 was not detected in the knockout clone E5KO. These results show that the clag9 gene product is not directly implicated in cytoadhesion to CD36.
Nacer A, Roux E, Pomel S, Scheidig-Benatar C, Sakamoto H, Lafont F, Scherf A, Mattei D. clag9 is not essential for PfEMP1 surface expression in non-cytoadherent Plasmodium falciparum parasites with a chromosome 9 deletion. PLoS One. 2011;6(12):e29039.
Other associated proteins
|PF3D7_0202000||knob-associated histidine-rich protein|
|PF3D7_0501500||rhoptry-associated protein 3|