Immuno-localization of GFP chimeras in transgenic parasites using a new Plasmodium falciparum cell fixation protocol. IspE(l)-GFP and PDF(f)-GFP were fixed and incubated with anti-ACP rabbit anti-serum. IspE(l)-GFP (A and B) co-localizes with anti-ACP. PDF(f)-GFP also co-localizes with anti-ACP (C and D), although it sometimes appears to localize within a sub-region of the ACP labelling (D). Stable CS(l)-GFP expressing cells (E and F) were incubated in the presence of anti-Hsp60 from E.coli. Overlay of GFP with Hsp60 suggests this Hsp60 PF10_0153 antibody is a useful mitochondrial marker. Transmission images were captured in both phase contrast and differential interference contrast (DIC) to highlight the integrity of cells fixed under these new conditions.
Tonkin CJ, van Dooren GG, Spurck TP, Struck NS, Good RT, Handman E, Cowman AF, McFadden GI. Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method. Mol Biochem Parasitol. 2004 137:13-21. Copyright Elsevier 2009.
Other associated proteins
|PF3D7_1022500||citrate synthase, mitochondrial, putative|