Sumoylation is differentially regulated during P. falciparum red blood cell stages. Indirect immunofluorescence microscopy of synchronized 3D7 parasites probed with anti-PfSUMO mAb 7E11 and anti-ACP antibodies. DNA was detected using DAPI. Bar indicates 2 μm. SUMO was concentrated in the nucleus of parasites at all three stages, with no discernible association with Maurer’s clefts. Consistent with immunoblot analysis, levels of detectable SUMO signal were greatest in trophozoite and schizont stage parasites.

Reiter K, Mukhopadhyay D, Zhang H, Boucher LE, Kumar N, Bosch J, Matunis MJ. Identification of Biochemically Distinct Properties of the Small Ubiquitin-related Modifier (SUMO) Conjugation Pathway in Plasmodium falciparum. J Biol Chem. 2013 Sep 27;288(39):27724-36.

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