Purity and integrity of isolated apicoplasts. (A) Protein samples from each purification step were examined by. (B) Immunofluorescence of intracellular trophozoite showing apicoplast with apicoplast stromal marker ACP (red), apicoplast outer membrane marker PfoTPT (green), and parasite nucleus (blue). (C) Purified apicoplasts bound to magnetic beads seen via anti-ACP (red) and anti-HA tagged TPT (green) labeling. (D–G) Electron micrographs showing purified apicoplasts in vicinity of magnetic beads (E), as confirmed by anti-PfoTPT immunogold labeling (E), four surrounding membranes (G), and ribosome-like particles within (G).

Botté CY, Yamaryo-Botté Y, Rupasinghe TW, Mullin KA, MacRae JI, Spurck TP, Kalanon M, Shears MJ, Coppel RL, Crellin PK, Maréchal E, McConville MJ, McFadden GI. Atypical lipid composition in the purified relict plastid (apicoplast) of malaria parasites. Proc Natl Acad Sci U S A. 2013 Apr 110(18):7506-11