Localization of PfARNP in free merozites and at the tight junction. (Upper panel) IFA of merozoite using anti PfARNP antibody. Co-staining of PfARNP (red) was performed with ASP, RON2 (1973–2067 aa) and AMA1 (green) in free merozoites. Staining was observed at the apex of merozoite distinct from nuclear stain DAPI (blue). The staining merged perfectly well with ASP, RON2 and not with AMA1 confirming the localization of PfARNP in neck of rhoptries of merozoites. Scale bar shows 1 mm. (Lower panel) Localization of PfARNP during invasion of merozoite. IFA of PfARNP was performed with RON2 and AMA1, known markers of tight junction in Cytochalasin D treated merozoites invading to erythrocytes. The labeling of PfARNP (red) was observed at the site of attachment of merozoite to erythrocyte. The staining of PfARNP merged perfectly with RON2 and AMA1(green), indicating that PfARNP is present at the site of tight junction. The inset of each image show the optically zoomed view of invading merozoite. Scale bar shows 2 mm.
Hans N, Singh S, Jain SK, Chauhan VS. Identification of novel rhoptry neck protein of Plasmodium falciparum. Mol Biochem Parasitol. 2013 188(1):34-9 Copyright Elsevier
Other associated proteins
|PF3D7_0511600||apical rhoptry neck protein|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1452000||rhoptry neck protein 2|