GFP fluorescence in parasites expressing GFP-2xFYVE. This construct has been used in various organisms to monitor the intracellular localization of PI3P. Colocalization of GFP-2xFYVE and the food vacuole membrane marker CRT using rabbit anti-CRT. Serial images of a Z-stack acquisition (0.38 mm step) are displayed. Arrowheads indicate protrusions from the food vacuole membrane that are colabeled with CRT and GFP-2xFYVE, while arrows indicate zones of intense GFP-2xFYVE staining in the absence of the food vacuole marker. A corresponding phase-contrast image is shown in the lower left corner. Fluorescence was associated mainly with the membrane of the food vacuole.

Tawk L, Chicanne G, Dubremetz JF, Richard V, Payrastre B, Vial HJ, Roy C, Wengelnik K. Phosphatidylinositol 3-phosphate, an essential lipid in Plasmodium, localizes to the food vacuole membrane and the apicoplast. Eukaryot Cell. 2010 9:1519-30.