PfEMP1 PfEMP1

Characterization of PfEMP1B-GFP at Maurer's clefts. (A) Transfectants (ITK strain) expressing the PfEMP1B-GFP chimera were co-labeled with BODIPY-ceramide and imaged using 3DSIM. Higher magnification images are displayed in the right column. PfEMP1B-GFP is present in horseshoe-shaped Maurer's clefts (aqua arrows). Spherical structures with no GFP labeling are marked with yellow arrows. (B) EqtII-permeabilized PfEMP1B-GFP transfectants were probed with antibodies recognizing GFP and REX1 and imaged by 3D-SIM. (C-F) EqtII-permeabilized 3D7 strain or PfEMP1B-GFP transfectants were labeled with anti-PfEMP1 ATS (C) or anti-GFP (D-F) and 6 nm Protein A-gold, and prepared for EM. A tether (t) and the parasite (P) are indicated. Scale bars = 2 μm (A, first three columns, B), 1 μm (A, last column), 500 nm (B, last column), 100 nm (C-F).

McMillan PJ, Millet C, Batinovic S, Maiorca M, Hanssen E, Kenny S, Muhle RA, Melcher M, Fidock DA, Smith JD, Dixon MW, Tilley L. Spatial and temporal mapping of the PfEMP1 export pathway in Plasmodium falciparum. Cell Microbiol. 2013 15(8):1401-18.

Other associated proteins

PFID Formal Annotation
PF3D7_0935900 ring-exported protein 1
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