Stage-specific localization of PfCS using confocal microscopy. The rows in each column represent four parasitic stages. First row (from the top), ring stage; second row, trophozoite; third row, schizont; fourth row, segmenter. The scale bars represent 1 mm. Left column (green), DNA stain with Hoechst dye; centre column (red), PfCS immunofluorescence with AbPfCS1; right column, composite. The appearance of a dispersed immunofluorescence pattern surrounding the nuclear stain and the absence of a discrete crescent-shaped signal clearly indicate that PfCS is cytosolic and not located to the apicoplast.

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