Localisation of GFP-tagged PfHK using confocal microscopy. Brightfield, GFP-fluorescence and merged images of erythrocytes infected with two ring-stage parasites (top) and a trophozoite-stage parasite (bottom). Scale bar represents approximately 1.5 mm. GFP-associated fluorescence (green) was diffuse throughout the parasite cytosol, both in the ring and trophozoite stages.
Tjhin ET, Staines HM, van Schalkwyk DA, Krishna S, Saliba KJ. Studies with the Plasmodium falciparum hexokinase reveal that PfHT limits the rate of glucose entry into glycolysis. FEBS Lett. 2013 587(19):3182-7