(B) Nucleoporin 100 (Nup100), a protein of the nuclear pore and BiP immunofluorescence analysis reveals the localization of Nup100 outside the DAPI stained area. Nup100 localizes in close proximity to BiP, a marker for the endoplasmic reticulum (ER) and does not overlap with DAPI, confirming that it defines the outer limits of the nucleus. (C) Localization of Nup100 and CenPA (histone 3) outside the DAPI stained area. CenPA and Nup100 do not co-localize; however, CenPA extends beyond the DAPI staining area of the nucleus suggesting that it is located within the periphery but inside the Nup100 delimited area of the nucleus (D) Localization of the histone marks H3K9m3 with CenPA and H3K9ac in the nucleus. The histone modification H3K9m3 silences transcription whereas H3K9ac is found associated with active transcription in general. H3K9m3 localized mostly to the nuclear periphery outside the DAPI stained area, which was consistent with the location of chromosome clusters containing silenced var genes. H3K9ac localized more with the DAPI stained area of the nucleus suggesting the DAPI stained area is broadly the transcriptionally active zone.Ea Trophozoite, Early Trophozoite; La trophozoite, Late Trophozoite.
Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40:109-21. Copyright Elsevier 2010.
Other associated proteins
|PF3D7_1333700||histone H3-like centromeric protein CSE4|