PF3D7_0905400 high molecular weight rhoptry protein 3

Immunoelectronmicroscopic studies were performed to confirm that the punctate appearance seen by indirect immunofluorescence was indeed due to localization of the target antigen in the rhoptries. Right: FC27 parasites obtained from asynchronous culture were sectioned and incubated with affinity purified human anti-Ag44 antibodies and subsequently with protein-A gold. Gold particles were localized within paired organelles at the apex of both immature merozoites within segmented schizonts (A) and free merozoites (B). These organelles had the typical appearance of rhoptries. Bottom: When ring-stage parasites were labelled with anti-Ag44 antibodies, label was found around the developing ring and scattered through the erythrocyte cytoplasm.

Coppel RL, Bianco AE, Culvenor JG, Crewther PE, Brown GV, Anders RF, Kemp DJ. A cDNA clone expressing a rhoptry protein of Plasmodium falciparum. Mol Biochem Parasitol. 1987 25:73-81. Copyright Elsevier 2010.

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