Parasite sections were incubated with rabbit anti-BiP serum and then labelled with anti-rabbit gold-conjugated IgG. (A) Untreated trophozoite stage showing gold particles lining the endoplasmic reticulum and the nuclear envelope. Note the connection between these organelles. N, nucleus; P, pigmented digestive vesicle; (*), endoplasmic reticulum. Scale bar represents 0.5 mm. (B) Brefeldin A-treated trophozoite stage. Compared with the control, the Brefeldin A-treated cells present a swollen endoplasmic reticulum. The inset shows the intense labelling of a cisternum with gold particles.
Nacer A, Berry L, Slomianny C, Mattei D. Plasmodium falciparum signal sequences: simply sequences or special signals? Int J Parasitol. 2001 31:1371-9. Copyright Elsevier