Localization of EEA1WT-mCherry as a marker for PI(3)P (phosphatidylinositol-3-phosphate) in the parasite ER. EEA1 = early endosomal antigen 1. Transgenic parasites transfected with EEA1 were fixed, permeabilized and probed with anti-mCherry as well as antibodies to endogenous P. falciparum markers (green) of the ER, like BIP (top row) and plasmepsin V (Pl. V, second row); a marker of the Golgi (ERD2, third row) or the PVM (Exp-2, bottom row). EEA1WT (red) is seen in punctuate ‘spots’ within the ER. Dotted circles show location of red cell membrane. Dotted squares show regions magnified in the right panels. Parasite nuclei were stained with Hoechst 33342 (blue). Scale bars, 5 mm. The overlap between markers and EEA1 indicates the presence of PI(3)P in the respective regions.
Bhattacharjee S, Stahelin RV, Speicher KD, Speicher DW, Haldar K. Endoplasmic Reticulum PI(3)P Lipid Binding Targets Malaria Proteins to the Host Cell. Cell. 2012 148(1-2):201-12.
Other associated proteins
|PF3D7_1323500||PEXEL protease plasmepsin V|
|PF3D7_1353600||ER lumen protein retaining receptor|
|PF3D7_1471100||exported protein 2|