Schizont and merozoite culture was incubated overnight with 0.1µM cytochalasin D to inhibit invasion. Thin smeared on a glass slide, fixed in methanol, stained with rabbit anit-GAP50 polyclonal serum at 1:200 and secondary Alexa-Flor488 anti-Rabbit. Dual color fluorescence images were captured using a Carl Zeiss Axioskop 2 microscope at 100x using Oil immersion. Nucleus is marked with DAPI. Scale bar indicate 1µM. Invading merozoite is shown with GAP50 clearly marking the periphery (shown to be the IMC by immuno-EM).
Baum J, Richard D, Healer J, Rug M, Krnajski Z, Gilberger TW, Green JL, Holder AA, Cowman AF. A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites. J Biol Chem. 2006 281(8):5197-208.