PfGAP50 is located in the ER prior to recruitment to the IMC. A. Parasites expressing PfGAP50-GFP (green) were incubated with ER-Tracker (depicted in red). The GFP and ER-Tracker fluorescence is co-located in trophozoite stage parasites. Upon relocation of GFP fluorescence to the apical ends of nascent merozoites during early schizont stage, the reticular ER-Tracker labelling persists. PfGAP50-GFP is concentrated at one pole of released merozoites while ER Tracker labels internal structures. B. Fixed cell immunofluorescence microscopy of the 3D7 parent strain labelled with antibodies raised against PfGAP50 (green) and PfERC (red). C. Immuno-fluorescence microscopy of PfGAP50-GFP transfectants at the mature and ruptured schizont stages labelled with anti-GFP (green), anti-PfGAP45 (red) and DAPI (blue). Bars = 1 μm. PfGAP50-GFP is located in the endoplasmic reticulum in early stage parasites, then redistributes to apical caps during the formation of daughter merozoites. In the final stage of schizogony the PfGAP50-GFP profile extends further around the merozoite surface
Yeoman JA, Hanssen E, Maier AG, Klonis N, Maco B, Baum J, Turnbull L, Whitchurch CB, Dixonand MW, Tilley L. Tracking glideosome-associated protein-50 reveals the development and organization of the inner membrane complex of P. falciparum. Eukaryot Cell. 2011 10(4):556-64.
Other associated proteins
|PF3D7_1108600||endoplasmic reticulum-resident calcium binding protein|
|PF3D7_1222700||glideosome-associated protein 45|