Localization of PfGAP proteins to the inner membrane complex. A, PfGAP50 and PfGAP45 co-localize with MTIP (a protein known to localize to the inner membrane complex) and partially with PfMyoA (known to associate with the innermembrane complex and merozoite apical tip (6, 38)). The white arrow in the MTIP inset points to an area of reduced fluorescence at the apical tip in a single z-stack, consistent with the absence of IMC at this position. B, immunoelectron microscopy confirms IMC localization for PfGAP45 (large 25-nm gold particle). The white arrows indicate trilaminar appearance of the IMC and merozoite plasma membrane. i, inner IMC membrane; o, outer IMC membrane; p, plasma membrane R, rhoptry. *, single anti-PfGAP50-conjugated gold particle found in close proximity to PfGAP45 but giving poor reactivity (typically 1 particle/merozoite). Scale bar in electron microscopy, 0.2 mm. C, PfGAP45-GFP shows fluorescence that concentrates to the merozoite periphery late in asexual development, consistent with the Scale bar in IFA, 1 mm.

Baum J, Richard D, Healer J, Rug M, Krnajski Z, Gilberger TW, Green JL, Holder AA, Cowman AF. A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites. J Biol Chem. 2006 281(8):5197-208.