Nucleosome assembly protein PFI0930c localizes to the far nuclear periphery. (A,B) PFI0930c is either tagged with GFP or Cherry, respectively. Co-localization studies by immunofluorescence reveal its localization to the nuclear periphery, which is marked by CenPA (histone H3)and delimited by the nuclear envelope marker Nup100 (A), but not to the cytoplasmic portion of the cell marked by aldolase (PF14_0425) and ERD2 (PF13_0280) (B). Co-localisation of PFI0930c-cherry and aldolase, used as a cytoplasmic marker showed clear separation of nuclear and cytoplasmic regions in the P. falciparum cell. Co-localisation analysis with Nup100 and ERD2, a marker for the cis- and trans-Golgi showed the same overlapping localisation of PFI0930c-cherry and Nup100, both in an internal location relative to ERD2.
Volz J, Carvalho TG, Ralph SA, Gilson P, Thompson J, Tonkin CJ, Langer C, Crabb BS, Cowman AF. Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum. Int J Parasitol. 2010 40:109-21. Copyright Elsevier 2010.
Other associated proteins
|PF3D7_0905100||nucleoporin NUP221, putative|
|PF3D7_0919000||nucleosome assembly protein|