Immunofluorescence microscopy was done as described under Section 2. (A) Thin smears were incubated with rabbit anti-ARP antibody. The secondary antibody was Cy2-conjugated (green), and nuclei were stained with DAPI (purple by computer color rendition). Fluorescence was viewed in a Nikon TE200 microscope equipped with DeltaVision software for deconvolution and reconstruction of 3D volume as described . As and where described, the ring, trophozoite, schizont, and segmented stages of the parasite were probed. (B) The secondary antibody was FITC-conjugated (green), and nuclei were stained with TO-PRO-3 iodide (red). A ring and a schizont are shown as representative examples. The protein was located mainly in the parasitic cytoplasm. Thus, PfARP is a unique cytoplasmic member of the SET/TAF-family and a candidate physiological regulator of the Plasmodium PP2A.
Dobson S, Kumar R, Bracchi-Ricard V, Freeman S, Al-Murrani SW, Johnson C, Damuni Z, Chakrabarti D, Barik S. Characterization of a unique aspartate-rich protein of the SET/TAF-family in the human malaria parasite, Plasmodium falciparum, which inhibits protein phosphatase 2A. Mol Biochem Parasitol. 2003 126:239-50. Copyright Elsevier 2009.