PF3D7_1203700 nucleosome assembly protein

Double immunofluorescence analysis of PfNAPL and PfNAPS in asexual and sexual parasites. Acetone fixed parasites were reacted with rabbit anti-NAPL antibodies and a rat anti-NAPS antibody (1:400). Anti-PfNAPL fluorescence (green, first column) and anti-PfNAPS fluorescence (red, second column) were obtained reacting the samples with fluorescein-conjugated anti rabbit IgG antiserum and rhodamine-conjugated anti rat IgG antiserum (1:200) (Cappel), and are combined with DAPI fluorescent signal. Composite picture of PfNAPL and PfNAPS fluorescent signals is shown in the “merge” column, in which DAPI fluorescence is not shown for clarity. Panel A: early rings; B: late rings; C: trophozoites/early schizonts; D: schizonts; E: free merozoites; Inserts at the right end in panels A and B contain higher magnification of an individual parasite at that time point. Bar is 5mm. PfNAPS did not co-localise with PfNAPL and was more intimately associated with the parasite nucleus,

Chandra BR, Olivieri A, Silvestrini F, Alano P, Sharma A. Biochemical characterization of the two nucleosome assembly proteins from Plasmodium falciparum. Mol Biochem Parasitol. 2005 142:237-47. Copyright Elsevier 2009.

Other associated proteins

PFID Formal Annotation
PF3D7_0919000 nucleosome assembly protein