Similar trafficking and subcellular localization of RhopH2-Myc-GFP (A–D) and RhopH2-GFP (E–H) chimeric proteins during late developmental stages of parasite growth. Images were collected from live parasites. (A and E) Early schizont stages showing almost even fluorescence signal in the cytoplasm and probably the parasitophorous vacuole. (B and F) Further developed schizonts showing accumulation of the chimeric proteins around the parasites. (C and G) Later schizont stages showing fluorescence surrounding individual merozoites. Fluorescent vesicles can be seen; probably representing premature rhoptries. (D and H) Segmented schizonts showing concentrated fluorescence signal at the apical end of the formed merozoites. Nuclei were stained with DAPI. Scale bar represents 5 μm.

Ghoneim A, Kaneko O, Tsuboi T, Torii M. The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries. Parasitol Int. 2007 56:31-43. Copyright Elsevier 2009.