Localization of proteins of PfMSP-1 associated complex by immunofluorescence assay. Unpermeabilized P. falciparum merozoites were coimmunostained with anti-PfRAP-1 and anti-PfMSP-142 antibodies (i), with anti-PfRhopH3-C and anti-PfMSP-142 antibodies (ii), with anti-PfRhopH3-C and anti-PfMSP-3 antibodies (iii), anti-PfRAP-1 and anti-PfMSP-3 antibodies (iv), and anti-Pffalcipain-2 and anti-PfMSP-142 antibodies (v). PfMSP-1 and PfMSP-3 staining was detected on the entire surface of merozoites, while PfRhopH3 and PfRAP-1 staining localized at the apical end of merozoites. Co-localization was observed between PfMSP-1 and PfRAP-1, PfMSP-1 and PfRhopH3, PfMSP-3 and PfRhopH3 as well PfMSP-1 and PfMSP-3 staining was detected on the entire surface of merozoites, while PfRhopH3 and PfRAP-1 staining localized at the apical end of merozoites. Co-localization was observed between PfMSP-1 and PfRAP-1, PfMSP-1 and PfRhopH3, PfMSP-3 and PfRhopH3 as well antibody and the specific location of PfRhopH3 in rhoptries.

Ranjan R, Chugh M, Kumar S, Singh S, Kanodia S, Hossain MJ, Korde R, Grover A, Dhawan S, Chauhan VS, Reddy VS, Mohmmed A, Malhotra P. Proteome analysis Rrveals a large merozoite surface protein-1 associated complex on the Plasmodium falciparum merozoite surface. J Proteome Res. 2010 10:680-91. Copyright American Chemical Society