PfRON5 localizes to the rhoptry neck. (A) Aotus antibodies against MSP-1 peptide 24148 (5501) displaying the membrane, and against RAMA peptide 36340 (33460),showing the rhoptries (red fluorescence), together with RON5 peptide 38556 (37742) (green fluorescence) and DAPI (blue fluorescence, merged at the bottom), confirming RON5 localization in the rhoptries but differently to that of RAMA. (B) Immuno-electron microscopy with rabbit antibodies against RON5 peptides and protein A-gold showed specific labeling in the P. falciparum merozoite rhoptry neck. PfRON5 was detected in pear-shaped rhoptry neck portion in segmented schizonts when using rabbit polyclonal antibodies for IEM assay.

Curtidor H, Patiño LC, Arévalo-Pinzón G, Vanegas M, Patarroyo ME, Patarroyo MA. Plasmodium falciparum rhoptry neck protein 5 peptides bind to human red blood cells and inhibit parasite invasion. Peptides. 2013 Aug 8. 9781(13)00268-4.