Wide field IFA time course of invasion using A) anti-MSP1/PfRON4. Scale bar = 2.0 μm. Before commencement of invasion, no loss of MSP1 labeling was apparent (MSP1 early). Loss of MSP1 tracked the exterior of invading merozoites concurrent with different stages of passage through the tight (MSP1 mid). The lack of contiguity between shed MSP1 and PfSUB2 sheddase, each in comparison with PfRON4 suggests that MSP1 may be processed by PfSUB2 early in invasion but shed only during passage through the junction.

Riglar DT, Richard D, Wilson DW, Boyle MJ, Dekiwadia C, Turnbull L, Angrisano F, Marapana DS, Rogers KL, Whitchurch CB, Beeson JG, Cowman AF, Ralph SA, Baum J. Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte. Cell Host Microbe. 2011 9:9-20.