VAR2CSA and CLAG9 are present in the IRBC membranes of C4S-adherent parasites. The C4S-adherent FCR3 parasites, at the late ring to early trophozoite stages, were analyzed by immunoelectron microscopy by using rat anti-VAR2CSA and rabbit anti-CLAG9 primary antibodies followed by biotin-conjugated goat anti-rat IgG and 18-nm gold particle-conjugated goat anti-rabbit IgG secondary antibodies. The bound biotin-labeled antibodies were probed with 12-nm gold particles conjugated streptavidin. Arrows and arrowheads indicate, respectively, 12-and 18-nm gold particles. (Scale bars: 200 nm.) a substantial level of CLAG9 is located mainly at or in close proximity of the IRBC membrane in association with VAR2CSA.
Goel S, Valiyaveettil M, Achur RN, Goyal A, Mattei D, Salanti A, Trenholme KR, Gardiner DL, Gowda DC. Dual stage synthesis and crucial role of cytoadherence-linked asexual gene 9 in the surface expression of malaria parasite var proteins. Proc Natl Acad Sci U S A. 2010 107:16643-8.
Other associated proteins
|PF3D7_1200600||erythrocyte membrane protein 1, PfEMP1|