PF3D7_0935900 ring-exported protein 1

Localization of the anti-Pf62 reactivity on asexual parasite stages by indirect immuno-fluorescence. Smears of parasite-infected red blood cells, strain Dd2, were reacted with rabbit anti-Pf62 polyclonal antisera. a Incubation with 1:50 dilution of antisera raised against the Pf62 protein. b Incubation with preimmune rabbit serum at the same dilution, derived from the same rabbit used to derive Pf62 antibodies prior to the first immunization. Ultrastructural localization of Pf62 protein in different stages. Transmission electron microscopy immunogold labeling (10 nm gold particles, arrows) in ultrathin sections. a General overview of parasite-infected RBCs (×6,300, original magnification). b The Pf62 protein is localized in the trophozoite and in the erythrocyte, both in the cell interior and surface. c The same trophozoite at higher magnification. Immunoscanning electron micrograph. Left The backscattered electron detector identifies the bright signals from the gold particles that localize the Pf62 protein. Right The same field using the secondary electron detector that shows the ultrastructure of the erythrocytes

Moyano EM, González LM, Montero E, Cuevas L, Perez-Pastrana E, Santa-Maria Y, Benito A. Initial characterization of Pf62, a novel protein of Plasmodium falciparum identified by immunoscreening. Parasitol Res. 2009 104:1389-97.

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